Simultaneous detection of monomers associated with resin-based dental composites using SPME and HPLC

As resin-based composites (RBC) replace dental amalgam for environmental reasons, there is a requirement to understand the impact of this alternative restorative material. In study we standardize simultaneous detection five monomeric components associated with RBCs using high performance liquid chromatography (HPLC) coupled solid-phase microextraction (SPME). Factors affecting method (detection wavelength, calibration conditions, sensitivity/accuracy/precision, extraction time/efficiency) are evaluated standard solutions containing mixture TEGDMA, UDMA, Bis-GMA, BPA and HEMA. Detection sensitivity analytical efficiency optimized these compounds 200 nm PDMS/DVB fiber time 90 min. Analytical accuracy HPLC >95% all monomers, precision 2.3–5.1%. limits under conditions described 25 µg/L HEMA, BPA, 100 TEGDMA. The governed by largest molecular weight compounds.